diff --git a/include/spdk/dif.h b/include/spdk/dif.h
index 17c3ade1f..e68d77654 100644
--- a/include/spdk/dif.h
+++ b/include/spdk/dif.h
@@ -366,4 +366,20 @@ int spdk_dif_verify_stream(struct iovec *iovs, int iovcnt,
 			   uint32_t data_offset, uint32_t data_len,
 			   struct spdk_dif_ctx *ctx,
 			   struct spdk_dif_error *err_blk);
+
+/**
+ * Calculate CRC-32C checksum of the specified range in the extended LBA payload.
+ *
+ * \param iovs iovec array describing the extended LBA payload.
+ * \param iovcnt Number of elements in the iovec array.
+ * \param data_offset Offset to the range
+ * \param data_len Length of the range
+ * \param crc32c Initial and updated CRC-32C value.
+ * \param ctx DIF context.
+ *
+ * \return 0 on success and negated errno otherwise.
+ */
+int spdk_dif_update_crc32c_stream(struct iovec *iovs, int iovcnt,
+				  uint32_t data_offset, uint32_t data_len,
+				  uint32_t *crc32c, const struct spdk_dif_ctx *ctx);
 #endif /* SPDK_DIF_H */
diff --git a/lib/util/dif.c b/lib/util/dif.c
index cfc13524c..a176ce021 100644
--- a/lib/util/dif.c
+++ b/lib/util/dif.c
@@ -1644,3 +1644,40 @@ spdk_dif_verify_stream(struct iovec *iovs, int iovcnt,
 error:
 	return rc;
 }
+
+int
+spdk_dif_update_crc32c_stream(struct iovec *iovs, int iovcnt,
+			      uint32_t data_offset, uint32_t data_len,
+			      uint32_t *_crc32c, const struct spdk_dif_ctx *ctx)
+{
+	uint32_t buf_len = 0, buf_offset = 0, len, offset_in_block;
+	uint32_t crc32c;
+	struct _dif_sgl sgl;
+	int rc;
+
+	if (iovs == NULL || iovcnt == 0) {
+		return -EINVAL;
+	}
+
+	crc32c = *_crc32c;
+	_dif_sgl_init(&sgl, iovs, iovcnt);
+
+	rc = _dif_sgl_setup_stream(&sgl, &buf_offset, &buf_len, data_offset, data_len, ctx);
+	if (rc != 0) {
+		return rc;
+	}
+
+	while (buf_len != 0) {
+		len = spdk_min(buf_len, _to_next_boundary(buf_offset, ctx->block_size));
+		offset_in_block = buf_offset % ctx->block_size;
+
+		crc32c = _dif_update_crc32c_split(&sgl, offset_in_block, len, crc32c, ctx);
+
+		buf_len -= len;
+		buf_offset += len;
+	}
+
+	*_crc32c = crc32c;
+
+	return 0;
+}
diff --git a/test/unit/lib/util/dif.c/dif_ut.c b/test/unit/lib/util/dif.c/dif_ut.c
index 9f066417b..ac7a7f624 100644
--- a/test/unit/lib/util/dif.c/dif_ut.c
+++ b/test/unit/lib/util/dif.c/dif_ut.c
@@ -2171,7 +2171,7 @@ update_crc32c_test(void)
 	_iov_alloc_buf(&iovs[3], 3 + 123);
 
 	/* data[1][511:123], md[1][5:0] */
-	_iov_alloc_buf(&iovs[4], 399 + 6);
+	_iov_alloc_buf(&iovs[4], 389 + 6);
 
 	/* md[1][7:6], data[2][511:0], md[2][7:0], data[3][431:0] */
 	_iov_alloc_buf(&iovs[5], 2 + 512 + 8 + 432);
@@ -2281,6 +2281,68 @@ _dif_update_crc32c_split_test(void)
 	free(buf);
 }
 
+static void
+dif_update_crc32c_stream_multi_segments_test(void)
+{
+	struct spdk_dif_ctx ctx = {};
+	struct iovec iov = {};
+	uint8_t *buf;
+	uint32_t dif_flags, crc32c1, crc32c2;
+	int rc;
+
+	dif_flags = SPDK_DIF_FLAGS_GUARD_CHECK | SPDK_DIF_FLAGS_APPTAG_CHECK |
+		    SPDK_DIF_FLAGS_REFTAG_CHECK;
+
+	rc = spdk_dif_ctx_init(&ctx, 4096 + 128, 128, true, false, SPDK_DIF_TYPE1,
+			       dif_flags, 22, 0xFFFF, 0x22, 0, GUARD_SEED);
+	CU_ASSERT(rc == 0);
+
+	buf = calloc(1, (4096 + 128) * 4);
+	SPDK_CU_ASSERT_FATAL(buf != NULL);
+	_iov_set_buf(&iov, buf, (4096 + 128) * 4);
+
+	rc = ut_data_pattern_generate(&iov, 1, 4096 + 128, 128, 4);
+	CU_ASSERT(rc == 0);
+
+	rc = spdk_dif_generate(&iov, 1, 4, &ctx);
+	CU_ASSERT(rc == 0);
+
+	crc32c1 = UT_CRC32C_XOR;
+	crc32c2 = UT_CRC32C_XOR;
+
+	/* 1st data segment */
+	_iov_set_buf(&iov, buf, 1024);
+	spdk_dif_ctx_set_data_offset(&ctx, 0);
+
+	rc = spdk_dif_update_crc32c_stream(&iov, 1, 0, 1024, &crc32c1, &ctx);
+	CU_ASSERT(rc == 0);
+
+	/* 2nd data segment */
+	_iov_set_buf(&iov, buf + 1024, (3072 + 128) + (4096 + 128) * 2 + 512);
+	spdk_dif_ctx_set_data_offset(&ctx, 1024);
+
+	rc = spdk_dif_update_crc32c_stream(&iov, 1, 0, 3072 + 4096 * 2 + 512, &crc32c1, &ctx);
+	CU_ASSERT(rc == 0);
+
+	/* 3rd data segment */
+	_iov_set_buf(&iov, buf + (4096 + 128) * 3 + 512, 3584 + 128);
+	spdk_dif_ctx_set_data_offset(&ctx, 4096 * 3);
+
+	rc = spdk_dif_update_crc32c_stream(&iov, 1, 0, 3584, &crc32c1, &ctx);
+	CU_ASSERT(rc == 0);
+
+	/* Update CRC32C for all data segments once */
+	_iov_set_buf(&iov, buf, (4096 + 128) * 4);
+	spdk_dif_ctx_set_data_offset(&ctx, 0);
+
+	rc = spdk_dif_update_crc32c(&iov, 1, 4, &crc32c2, &ctx);
+	CU_ASSERT(rc == 0);
+
+	CU_ASSERT(crc32c1 == crc32c2);
+
+	free(buf);
+}
+
 int
 main(int argc, char **argv)
 {
@@ -2379,7 +2441,9 @@ main(int argc, char **argv)
 			    dif_verify_stream_multi_segments_test) == NULL ||
 		CU_add_test(suite, "update_crc32c_test", update_crc32c_test) == NULL ||
 		CU_add_test(suite, "_dif_update_crc32c_split_test",
-			    _dif_update_crc32c_split_test) == NULL
+			    _dif_update_crc32c_split_test) == NULL ||
+		CU_add_test(suite, "dif_update_crc32c_stream_multi_segments_test",
+			    dif_update_crc32c_stream_multi_segments_test) == NULL
 	) {
 		CU_cleanup_registry();
 		return CU_get_error();